Key features and details
- Rabbit polyclonal to ROX
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ROX antibody
See all ROX primary antibodies
DescriptionRabbit polyclonal to ROX
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human ROX (internal sequence). The exact sequence is proprietary.
Database link: Q8IUF8
- WB: Raji whole cell lysate. IHC-P: HBL435 xenograft tissue. ICC/IF: A431 cells.
This product was previously labelled as MINA53
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 53 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Tissue specificityExpressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
Sequence similaritiesBelongs to the MINA53/NO66 family.
Contains 1 JmjC domain.
Cellular localizationNucleus. Nucleus > nucleolus.
- Information by UniProt
- 60S ribosomal protein L27a histidine hydroxylase antibody
- Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
- FLJ14393 antibody
Anti-ROX antibody (ab229117) at 1/3000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Paraffin-embedded HBL435 xenograft tissue stained for ROX with ab229117 at 1/100 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for ROX (green) using ab229117 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229117 has not yet been referenced specifically in any publications.