Overview

  • Product name

  • Description

    Rabbit polyclonal to ROX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ROX (internal sequence). The exact sequence is proprietary.
    Database link: Q8IUF8

  • Positive control

    • WB: Raji whole cell lysate. IHC-P: HBL435 xenograft tissue. ICC/IF: A431 cells.
  • General notes

     This product was previously labelled as MINA53

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • Tissue specificity

    Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • Sequence similarities

    Belongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • Cellular localization

    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

Images

  • Anti-ROX antibody (ab229117) at 1/3000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 53 kDa



    10% SDS-PAGE

  • Paraffin-embedded HBL435 xenograft tissue stained for ROX with ab229117 at 1/100 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for ROX (green) using ab229117 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229117.
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