Overview

  • Product name

    Anti-ROX antibody [EPR11721]
    See all ROX primary antibodies
  • Description

    Rabbit monoclonal [EPR11721] to ROX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    within Human ROX aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8IUF8

  • Positive control

    • A431, Raji, Jurkat and HeLa whole cell lysate (ab150035). A431 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     This product was previously labelled as MINA53

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11721
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
    • Tissue specificity

      Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
    • Sequence similarities

      Belongs to the MINA53/NO66 family.
      Contains 1 JmjC domain.
    • Cellular localization

      Nucleus. Nucleus > nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 60S ribosomal protein L27a histidine hydroxylase antibody
      • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
      • FLJ14393 antibody
      • Histone lysine demethylase MINA antibody
      • MDIG antibody
      • MINA antibody
      • MINA_HUMAN antibody
      • Mineral dust-induced gene protein antibody
      • Myc induced nuclear antigen 53 kDa antibody
      • MYC induced nuclear antigen antibody
      • MYC-induced nuclear antigen antibody
      • NO52 antibody
      • Nucleolar protein 52 antibody
      • Ribosomal oxygenase MINA antibody
      • ROX antibody
      see all

    Images

    • All lanes : Anti-ROX antibody [EPR11721] (ab173573) at 1/1000 dilution

      Lane 1 : A431 cell lysates
      Lane 2 : Raji cell lysates
      Lane 3 : Jurkat cell lysates
      Lane 4 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa

    • Immunofluorescence analysis of A431 cells labeling ROX with ab173573 at a 1/100 dilution.

    References

    ab173573 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173573.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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