Anti-RP105 antibody (ab126267)
Key features and details
- Rabbit polyclonal to RP105
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RP105 antibody
See all RP105 primary antibodies -
Description
Rabbit polyclonal to RP105 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 195-503 of Human RP105 (Q99467).
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Positive control
- HL60 whole cell lysate; CL15 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. -
Tissue specificity
Expressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 19 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4064 Human
- Omim: 602226 Human
- SwissProt: Q99467 Human
- Unigene: 87205 Human
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Alternative names
- CD180 antibody
- CD180 antigen antibody
- CD180_HUMAN antibody
see all
Images
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Western blot - Anti-RP105 antibody (ab126267)Anti-RP105 antibody (ab126267) at 1/1000 dilution + HL60 whole cell lysate at 30 µg
Predicted band size: 74 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody (ab126267)ab126267, at 1/500 dilution, staining RP105 in CL15 xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126267 has not yet been referenced specifically in any publications.