Key features and details
- Rabbit polyclonal to RP105
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RP105 antibody
See all RP105 primary antibodies
DescriptionRabbit polyclonal to RP105
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic 14 amino acid peptide from near the carboxy terminus of human RP105 .
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 74 kDa.|
FunctionMay cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells.
Tissue specificityExpressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 19 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD180 antibody
- CD180 antigen antibody
- CD180_HUMAN antibody
Immunohistochemistry of RP105 in human spleen tissue with ab45429 at 10 µg/ml.
Lane 1 : Anti-RP105 antibody (ab45429) at 0.5 µg/ml
Lane 2 : Anti-RP105 antibody (ab45429) at 1 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 74 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
ab45429 has been referenced in 1 publication.
- Bastiaansen AJ et al. TLR4 Accessory Molecule RP105 (CD180) Regulates Monocyte-Driven Arteriogenesis in a Murine Hind Limb Ischemia Model. PLoS One 9:e99882 (2014). IHC-P, IHC-Fr ; Mouse . PubMed: 24945347