Product nameAnti-RP105 antibody
See all RP105 primary antibodies
DescriptionRabbit polyclonal to RP105
Tested applicationsSuitable for: IHC-Fr, IHC-P, IHC (PFA fixed), WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic 14 amino acid peptide from near the carboxy terminus of human RP105 .
- Human spleen tissue, human spleen tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24945347|
|IHC-P||Use a concentration of 10 µg/ml.|
|IHC (PFA fixed)||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 74 kDa.|
FunctionMay cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells.
Tissue specificityExpressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 19 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD180 antibody
- CD180 antigen antibody
- CD180_HUMAN antibody
Immunohistochemistry of RP105 in human spleen tissue with ab45429 at 10 µg/ml.
Lane 1 : Anti-RP105 antibody (ab45429) at 0.5 µg/ml
Lane 2 : Anti-RP105 antibody (ab45429) at 1 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 74 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?