Product nameAnti-RP2 antibody [EPR11362]
See all RP2 primary antibodies
DescriptionRabbit monoclonal [EPR11362] to RP2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: IF,IHC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RP2 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O75695
- HeLa and Sh-SY5Y cell lysates; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 40 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceFunction: Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins. Tissue specificity: Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level). Disease: Retinitis pigmentosa 2 Similarity: Belongs to the TBCC family. Contains 1 C-CAP/cofactor C-like domain. PTM: Myristoylated on Gly-2; which may be required for membrane targeting. Palmitoylated on Cys-3; which may be required for plasma membrane targeting (Probable). Mutation of Cys-3 targets the protein to internal membranes.
Cellular localizationCell membrane; Lipid-anchor; Cytoplasmic side
- DELXp11.3 antibody
- KIAA0215 antibody
- NM23 H10 antibody
All lanes : Anti-RP2 antibody [EPR11362] (ab174840) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Sh-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
Flow cytometric analysis of permeabilized HeLa cells labeling RP2 with ab174840 at a 1/100 dilution (red), or a rabbit IgG (negative) (green).
ab174840 has not yet been referenced specifically in any publications.