Product nameAnti-RPA14/RPA3 antibody
See all RPA14/RPA3 primary antibodies
DescriptionRabbit polyclonal to RPA14/RPA3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment corresponding to Human RPA14/RPA3 aa 1-91.
Database link: NP_002938
- WB: H1299, HeLaS3, MOLT4 and HepG2 whole cell lysate (ab7900) IHC-P: Human lung CA patient tumor
This product was previously labelled as RPA14
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 14 kDa.|
|IHC-P||1/100 - 1/250.|
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
- Information by UniProt
- p14 antibody
- REPA 3 antibody
- REPA3 antibody
All lanes : Anti-RPA14/RPA3 antibody (ab97436) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa
Immunohistochemical analysis of RPA14/RPA3 in paraffin embedded lung CA patient tumor, using ab97436 at a 1/100 dilution.
ab97436 has not yet been referenced specifically in any publications.