Overview

  • Product name

  • Description

    Rabbit polyclonal to RPA14/RPA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human RPA14/RPA3 aa 1-91.
    Database link: NP_002938

  • Positive control

    • WB: H1299, HeLaS3, MOLT4 and HepG2 whole cell lysate (ab7900) IHC-P: Human lung CA patient tumor
  • General notes

     This product was previously labelled as RPA14

     

Properties

Applications

Our Abpromise guarantee covers the use of ab97436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function

    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • p14 antibody
    • REPA 3 antibody
    • REPA3 antibody
    • Replication factor A protein 3 antibody
    • Replication protein A 14 kDa subunit antibody
    • Replication protein A3 14kD antibody
    • Replication protein A3 14kDa antibody
    • Replication protein A3 antibody
    • RF A antibody
    • RF A protein 3 antibody
    • RF-A protein 3 antibody
    • RFA protein 3 antibody
    • RFA3_HUMAN antibody
    • RP A antibody
    • RP A p14 antibody
    • RP-A p14 antibody
    • RPA 14 antibody
    • RPA 3 antibody
    • RPA p14 antibody
    • rpa3 antibody
    see all

Images

  • All lanes : Anti-RPA14/RPA3 antibody (ab97436) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 14 kDa

  • Immunohistochemical analysis of RPA14/RPA3 in paraffin embedded lung CA patient tumor, using ab97436 at a 1/100 dilution.

References

ab97436 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab97436.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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