Product nameAnti-Rpa2 (phospho S4 + S8) antibody
DescriptionRabbit polyclonal to Rpa2 (phospho S4 + S8)
Tested applicationsSuitable for: IP, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Synthetic peptide within Human Rpa2 (phospho S4 + S8). The exact sequence is proprietary. NP_002937.1
Database link: P15927
- WB: HeLa cell lysate treated with 100 µM etoposide. IHC-P: Human lung cancer and mouse colon carcinoma tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264116 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|ICC/IF||1/500 - 1/5000.|
|WB||1/2000 - 1/10000.|
- REPA2 antibody
- replication protein A2 (32kD) antibody
- replication protein A2 32kDa antibody
- RPA32 antibody
Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right) labeling Rpa2 using ab264116 at 1/1000 dilution (Red). A Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstained with an Alex Fluor® phalloidin (Green).
All lanes : Anti-Rpa2 (phospho S4 + S8) antibody (ab264116) at 0.1 µg/ml
Lane 1 : HeLa cells treated with 100 µM etoposide (+)
Lane 2 : HeLa cells mock treated (-)
Lysates/proteins at 50 µg per lane.
Detection: Chemiluminescence with an exposure time of 75 seconds.
Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with etoposide and/or Lambda PP or mock treated. RPA32 (S4/S8) was immunoprecipitated using a different rabbit anti-RPA32 antibody. For blotting immunoprecipitated Phospho RPA32 (S4/S8), ab264116 was used at 0.1 μg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Lane 1: ab264116 treated with etoposide and Lambda PP.
Lane 2: ab264116 treated with etopside.
Lane 3: Control IgG treated with etopside.
Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB
ab264116 has not yet been referenced specifically in any publications.