Product nameAnti-RPA32/RPA2 antibody [2927C5]
See all RPA32/RPA2 primary antibodies
DescriptionMouse monoclonal [2927C5] to RPA32/RPA2
Tested applicationsSuitable for: Flow Cyt, Dot blot, WBmore details
Species reactivityReacts with: Human
Recombinant fragment: C terminal human RPA2.
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.1312% Sodium phosphate, 1.05% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab51499 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab51499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.5µg
WB: 1/200. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
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Anti-RPA32/RPA2 antibody [2927C5] (ab51499) at 1/200 dilution + HeLa whole cell lysate at 25 µg
Anti Mouse IgG at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Flow cytometry using ab51499 at 0.5ug. Secondary used was an AlexaFluor® 488 conjugated Goat Anti-mouse IgG (0.25 µg)
ab51499 has not yet been referenced specifically in any publications.