Anti-RPA32/RPA2 antibody (ab10359)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-RPA32/RPA2 antibody
See all RPA32/RPA2 primary antibodies -
DescriptionRabbit polyclonal to RPA32/RPA2
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant
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Immunogen
Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human replication protein A2, 32 kDa subunit (LocusLink ID 6118).
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Positive control
- Whole cell extract from HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab10359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
Post-translational
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
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Database links
- Entrez Gene: 456680 Chimpanzee
- Entrez Gene: 101144875 Gorilla
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 100347550 Rabbit
- Entrez Gene: 716612 Rhesus monkey
- Omim: 179836 Human
- SwissProt: P15927 Human
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Alternative names
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)IHC image of RPA32/RPA2 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10359, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-RPA32/RPA2 antibody (ab10359)ab10359 detection of RPA32 by Western Blot. Samples: Whole cell extract (75 µg) from asynchronously growing HeLa cells treated with 10 J/m2 UV radiation for the indicated period of time.ab10359 detection of RPA32 by Western Blot. Samples: Whole cell extract (75
µ g) from asynchronously growing HeLa cells treated with 10 J/m2 UV radiation for the indicated period of time.
References
This product has been referenced in:
- Dai J et al. Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis. Cell Rep 18:1383-1394 (2017). Read more (PubMed: 28178517) »
- Hashimoto Y et al. Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis. Nat Struct Mol Biol 17:1305-11 (2010). Read more (PubMed: 20935632) »
