Anti-RPA32/RPA2 antibody (ab10359)
Key features and details
- Rabbit polyclonal to RPA32/RPA2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPA32/RPA2 antibody
See all RPA32/RPA2 primary antibodies -
Description
Rabbit polyclonal to RPA32/RPA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human replication protein A2, 32 kDa subunit (LocusLink ID 6118).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Rabbit
Horse
Pig
Chimpanzee
Rhesus monkey
Gorilla
Bat
Elephant
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
|
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| IP |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
|
WB
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
|
IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
Post-translational
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
Cellular localization
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 456680 Chimpanzee
- Entrez Gene: 101144875 Gorilla
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 100347550 Rabbit
- Entrez Gene: 716612 Rhesus monkey
- Omim: 179836 Human
- SwissProt: P15927 Human
see all -
Alternative names
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
Images
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Western blot - Anti-RPA32/RPA2 antibody (ab10359)All lanes : Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 whole cell lysate
Lane 5 : Mouse NIH 3T3 whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)HeLa whole cell lysate cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-).
Affinity purified rabbit anti-RPA32 antibody (3 µg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.
Detection: Chemiluminescence
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab10359 has been referenced in 5 publications.
- Hirota T et al. SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice. Dev Cell 47:645-659.e6 (2018). PubMed: 30393076
- Tubbs A et al. Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork Collapse. Cell 174:1127-1142.e19 (2018). PubMed: 30078706
- Dai J et al. Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis. Cell Rep 18:1383-1394 (2017). PubMed: 28178517
- Hashimoto Y et al. Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis. Nat Struct Mol Biol 17:1305-11 (2010). PubMed: 20935632
- Guervilly JH et al. Loss of CHK1 function impedes DNA damage-induced FANCD2 monoubiquitination but normalizes the abnormal G2 arrest in Fanconi anemia. Hum Mol Genet 17:679-89 (2008). PubMed: 18029388