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    rpa32rpa2-antibody-ab10359.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response DNA Damage Recognition
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Anti-RPA32/RPA2 antibody (ab10359)

  • Datasheet
  • SDS
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Western blot - Anti-RPA32/RPA2 antibody (ab10359)
  • Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)

Key features and details

  • Rabbit polyclonal to RPA32/RPA2
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RPA32/RPA2 antibody
    See all RPA32/RPA2 primary antibodies
  • Description

    Rabbit polyclonal to RPA32/RPA2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human replication protein A2, 32 kDa subunit (LocusLink ID 6118).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human RPA32/RPA2 protein (ab101216)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
WB
Human
All applications
Rabbit
Horse
Pig
Chimpanzee
Rhesus monkey
Gorilla
Bat
Elephant
Application Abreviews Notes
WB
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Notes
WB
1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    modifications

    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localization

    Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Target information above from: UniProt accession P15927 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 456680 Chimpanzee
    • Entrez Gene: 101144875 Gorilla
    • Entrez Gene: 6118 Human
    • Entrez Gene: 19891 Mouse
    • Entrez Gene: 100347550 Rabbit
    • Entrez Gene: 716612 Rhesus monkey
    • Omim: 179836 Human
    • SwissProt: P15927 Human
    • SwissProt: Q62193 Mouse
    • Unigene: 703070 Human
    • Unigene: 79411 Human
    • Unigene: 2870 Mouse
    see all
  • Alternative names

    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Images

  • Western blot - Anti-RPA32/RPA2 antibody (ab10359)
    Western blot - Anti-RPA32/RPA2 antibody (ab10359)
    All lanes : Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HEK293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Mouse TCMK-1 whole cell lysate
    Lane 5 : Mouse NIH 3T3 whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 32 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)
    Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)

    HeLa whole cell lysate cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-).

    Affinity purified rabbit anti-RPA32 antibody (3 µg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.

    Detection: Chemiluminescence 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab10359? Please let us know so that we can cite the reference in this datasheet.

ab10359 has been referenced in 5 publications.

  • Hirota T  et al. SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice. Dev Cell 47:645-659.e6 (2018). PubMed: 30393076
  • Tubbs A  et al. Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork Collapse. Cell 174:1127-1142.e19 (2018). PubMed: 30078706
  • Dai J  et al. Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis. Cell Rep 18:1383-1394 (2017). PubMed: 28178517
  • Hashimoto Y  et al. Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis. Nat Struct Mol Biol 17:1305-11 (2010). PubMed: 20935632
  • Guervilly JH  et al. Loss of CHK1 function impedes DNA damage-induced FANCD2 monoubiquitination but normalizes the abnormal G2 arrest in Fanconi anemia. Hum Mol Genet 17:679-89 (2008). PubMed: 18029388

Customer reviews and Q&As

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