RecombinantRabMAb

Recombinant Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Reviews (1)Q&A (1)
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
  • Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
  • Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
  • Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
  • Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2876(2)] to RPA32/RPA2
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-RPA32/RPA2 antibody [EPR2876(2)]
    See all RPA32/RPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2876(2)] to RPA32/RPA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human RPA32/RPA2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HUVEC, HepG2, and MCF7 cell lysates; IHC-P: Human colon and Human ovary carcinoma tissues. ICC/IF: C6 and HeLa cells Flow Cyt: C6 and HeLa cells

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
      Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

    • Post-translational
      modifications

      Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.

    • Cellular localization

      Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt

    • Database links

      • Alternative names

        • 60S acidic ribosomal protein P1 antibody
        • AA409079 antibody
        • AI325195 antibody
        • AU020965 antibody
        • ik:tdsubc_2g1 antibody
        • M(2)21C antibody
        • MGC137236 antibody
        • OTTHUMP00000004008 antibody
        • p32 antibody
        • p34 antibody
        • RCJMB04_6d17 replication protein A2, 32kDa antibody
        • REPA2 antibody
        • Replication factor A protein 2 antibody
        • Replication protein A 32 kDa subunit antibody
        • Replication protein A 32kDa subunit antibody
        • Replication protein A 34 kDa subunit antibody
        • Replication protein A antibody
        • Replication Protein A2 (32kDa) antibody
        • Replication protein A2 antibody
        • Replication protein A2, 32kDa antibody
        • RF-A protein 2 antibody
        • Rf-A2 antibody
        • RFA antibody
        • RFA2_HUMAN antibody
        • RP-A p32 antibody
        • RP-A p34 antibody
        • RP21C antibody
        • RPA 2 antibody
        • RPA 32 antibody
        • RPA antibody
        • Rpa2 antibody
        • RPA32 antibody
        • RPA34 antibody
        • RpLP1 antibody
        • RpP2 antibody
        • xx:tdsubc_2g1 antibody
        • zgc:109822 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      • Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      • Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:460 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
      • Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:460 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
      • Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        All lanes : Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HUVEC cell lysate
        Lane 3 : HepG2 cell lysate
        Lane 4 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 29 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

        ab109084 at 1/250 dilution staining RPA32/RPA2 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

        ab109084 at 1/250 dilution staining RPA32/RPA2 in Human ovary carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)
        Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

      References (0)

      Publishing research using ab109084? Please let us know so that we can cite the reference in this datasheet.

      ab109084 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Question

      Customer kindly contacted us to inquire about RPA32/RPA2 antibodies and testing of ab109084 in ICC/IF.

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      Answer

      I am very pleased to hear you would like to accept our offer and test ab109084 inICC/IF. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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