Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: .5% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 20mM Tris pH7.5 with BSA/Tween
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L), DyLight 594 Conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 594
Dilution
1/1000
Additional data
Additional Notes
The antibody at higher concentrations showed what I would expect for RPA staining, i.e. nuclear staining. But I also saw clusters of staining outside of the nucleus which was unexpected.
Abcam user community
Verified customer
Submitted Aug 30 2012