Key features and details
- Rabbit polyclonal to RPA32/RPA2 (phospho S33)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPA32/RPA2 (phospho S33) antibody
See all RPA32/RPA2 primary antibodies
DescriptionRabbit polyclonal to RPA32/RPA2 (phospho S33)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human RPA32/RPA2 (phospho S33). (NP_002937.1)
Database link: P15927
- HeLa whole cell lysate treated with 100 µM etoposide for 4 hours.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
Numerous bands representing proteins that migrate more slowly than 60 to 70 kDa have been observed during WB. Under some conditions, at least two of these may be more intense than the band that represents phospho RPA32/RPA2 (S33).
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
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All lanes : Anti-RPA32/RPA2 (phospho S33) antibody (ab211877) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate treated with 100 µM etoposide for 4 hours
Lane 2 : HeLa whole cell lysate mock treated
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 seconds
Lower panel shows Western Blotting for total RPA32/RPA2 using an affinity purified rabbit anti-RPA32/RPA2 antibody at 0.1 µg/ml with an exposure time of 10 seconds.
ab211877 has been referenced in 2 publications.
- Amato R et al. G-quadruplex Stabilization Fuels the ALT Pathway in ALT-positive Osteosarcoma Cells. Genes (Basel) 11:N/A (2020). PubMed: 32183119
- Edwards DS et al. BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation. Cell Rep 32:108166 (2020). PubMed: 32966794