Product nameAnti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1]
See all RPA32/RPA2 primary antibodies
DescriptionRabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8)
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human RPA32/RPA2 (phospho S4 + S8). Represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
Database link: P15927
Dually phosphorylated synthetic peptide surrounding serine 4/8, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
This product is sold under License from Bethyl Laboratories, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 5µl/0.5mg lysate.
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
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pRPA32 (S4/S8) was immunoprecipitated from 1 mg HeLa whole cell lysate treated with 100 µM etoposide for 4 hours or untreated, using ab243866 at 5 µl per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.
Lane 1: ab243866 IP in HeLa cell lysate treated with 100 µM etoposide.
Lane 2: ab243866 Ip in untreated Hela whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate treated with 100 µM etoposide.
Detection: Chemiluminescence with an exposure time of 3 minutes
Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.
Lane 1: HeLa cell lysate treated with 100 µM etoposide for 4 hours.
Lane 2: HeLa cell lysate negetive treatment.A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab243866 has not yet been referenced specifically in any publications.