Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8)
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1]
See all RPA32/RPA2 primary antibodies
DescriptionRabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8)
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human RPA32/RPA2 (phospho S4 + S8). Represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
Database link: P15927
Dually phosphorylated synthetic peptide surrounding serine 4/8, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
This product is sold under License from Bethyl Laboratories, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 5µl/0.5mg lysate.
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
pRPA32 (S4/S8) was immunoprecipitated from 1 mg HeLa whole cell lysate treated with 100 µM etoposide for 4 hours or untreated, using ab243866 at 5 µl per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.
Lane 1: ab243866 IP in HeLa cell lysate treated with 100 µM etoposide.
Lane 2: ab243866 Ip in untreated Hela whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate treated with 100 µM etoposide.
Detection: Chemiluminescence with an exposure time of 3 minutes
Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.
Lane 1: HeLa cell lysate treated with 100 µM etoposide for 4 hours.
Lane 2: HeLa cell lysate negetive treatment.A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243866 has not yet been referenced specifically in any publications.