Overview

  • Product name

    Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1]
    See all RPA32/RPA2 primary antibodies
  • Description

    Rabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RPA32/RPA2 (phospho S4 + S8). Represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
    Database link: P15927

  • General notes

    Dually phosphorylated synthetic peptide surrounding serine 4/8, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab243866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP Use at an assay dependent concentration.

Use 5µl/0.5mg lysate.

Target

  • Function

    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    modifications

    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localization

    Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Images

  • pRPA32 (S4/S8) was immunoprecipitated from 1 mg HeLa whole cell lysate treated with 100 µM etoposide for 4 hours or untreated, using ab243866 at 5 µl per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.

    Lane 1: ab243866 IP in HeLa cell lysate treated with 100 µM etoposide.

    Lane 2: ab243866 Ip in untreated Hela whole cell lysate.

    Lane 3: Control IgG in HeLa whole cell lysate treated with 100 µM etoposide.

    Detection: Chemiluminescence with an exposure time of 3 minutes

  • Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.

    Lane 1: HeLa cell lysate treated with 100 µM etoposide for 4 hours.

    Lane 2: HeLa cell lysate negetive treatment.

    A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab243866 has not yet been referenced specifically in any publications.

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