Key features and details
- Rabbit polyclonal to RPA32/RPA2 (phospho T21)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPA32/RPA2 (phospho T21) antibody
See all RPA32/RPA2 primary antibodies
DescriptionRabbit polyclonal to RPA32/RPA2 (phospho T21)
Specificityab61065 detects endogenous levels of RPA32/RFA2 only when phosphorylated at threonine 21.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human RPA32/RPA2. The exact sequence is proprietary. Synthesized phosphopeptide derived from human RPA32/RFA2 around the phosphorylation site of threonine 21(G-Y-TP-Q-S).
Database link: P15927
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab61065 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
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All lanes : Anti-RPA32/RPA2 (phospho T21) antibody (ab61065) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours with immunizing phosphopeptide
Developed using the ECL technique.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab61065 at 1/50 dilution.
Left panel: Without immunizing phosphopeptide.
Right panel: Plus immunizing phosphopeptide.
ab61065 has been referenced in 18 publications.
- Zhang G et al. Chromatin remodeler HELLS maintains glioma stem cells through E2F3 and MYC. JCI Insight 4:N/A (2019). PubMed: 30779712
- Ibler AEM et al. Typhoid toxin exhausts the RPA response to DNA replication stress driving senescence and Salmonella infection. Nat Commun 10:4040 (2019). PubMed: 31492859
- Lai Y et al. HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation. Sci Rep 9:14257 (2019). PubMed: 31582797
- Juhász S et al. ATRX Promotes DNA Repair Synthesis and Sister Chromatid Exchange during Homologous Recombination. Mol Cell 71:11-24.e7 (2018). PubMed: 29937341
- Maya-Mendoza A et al. High speed of fork progression induces DNA replication stress and genomic instability. Nature 559:279-284 (2018). PubMed: 29950726