Overview

  • Product name

    Anti-RPA32/RPA2 (phospho T21) antibody
    See all RPA32/RPA2 primary antibodies
  • Description

    Rabbit polyclonal to RPA32/RPA2 (phospho T21)
  • Host species

    Rabbit
  • Specificity

    ab61065 detects endogenous levels of RPA32/RFA2 only when phosphorylated at threonine 21.
  • Tested applications

    Suitable for: WB, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RPA32/RPA2. The exact sequence is proprietary. Synthesized phosphopeptide derived from human RPA32/RFA2 around the phosphorylation site of threonine 21(G-Y-TP-Q-S).
    Database link: P15927

  • Positive control

    • IHC-P: Human lung carcinoma tissue. WB: Adriamycin treated HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab61065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22773103
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Function

    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    modifications

    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localization

    Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Images

  • All lanes : Anti-RPA32/RPA2 (phospho T21) antibody (ab61065) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours with immunizing phosphopeptide

    Developed using the ECL technique.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab61065 at 1/50 dilution.

    Left panel: Without immunizing phosphopeptide.

    Right panel: Plus immunizing phosphopeptide.

References

This product has been referenced in:

  • Zhang G  et al. Chromatin remodeler HELLS maintains glioma stem cells through E2F3 and MYC. JCI Insight 4:N/A (2019). Read more (PubMed: 30779712) »
  • Juhász S  et al. ATRX Promotes DNA Repair Synthesis and Sister Chromatid Exchange during Homologous Recombination. Mol Cell 71:11-24.e7 (2018). Read more (PubMed: 29937341) »
See all 16 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (A549 tumour cell line)
Loading amount
50 µg
Specification
A549 tumour cell line
Treatment
1h with DNA-PK inhibitor +/- 10IU Bleomycin (4h)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 11 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up