Key features and details
- Rabbit polyclonal to RPA32/RPA2 (phospho T21) - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPA32/RPA2 (phospho T21) antibody - N-terminal
See all RPA32/RPA2 primary antibodies
DescriptionRabbit polyclonal to RPA32/RPA2 (phospho T21) - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Zebrafish, Rhesus monkey
Synthetic peptide within Human RPA32/RPA2 (N terminal) (phospho T21). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P15927
- WB: HEK-293T whole cell extract.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
All lanes : Anti-RPA32/RPA2 (phospho T21) antibody - N-terminal (ab229459) at 1/1000 dilution
Lane 1 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract
Lane 2 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract treated with 100 µM Etoposide for 2 hours
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229459 has not yet been referenced specifically in any publications.