Product nameAnti-RPA32/RPA2 (phospho T21) antibody - N-terminal
See all RPA32/RPA2 primary antibodies
DescriptionRabbit polyclonal to RPA32/RPA2 (phospho T21) - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Zebrafish, Rhesus monkey
Synthetic peptide within Human RPA32/RPA2 (N terminal) (phospho T21). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P15927
- WB: HEK-293T whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
All lanes : Anti-RPA32/RPA2 (phospho T21) antibody - N-terminal (ab229459) at 1/1000 dilution
Lane 1 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract
Lane 2 : HEK293T (human epithelial cell line from embryonic kidney) whole cell extract treated with 100 µM Etoposide for 2 hours
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS-PAGE gel.
ab229459 has not yet been referenced specifically in any publications.