Anti-RPA4 antibody - C-terminal (ab200742)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RPA4 antibody - C-terminal -
Description
Rabbit polyclonal to RPA4 - C-terminal -
Host species
Rabbit -
Specificity
Detects endogenous levels of RPA4 protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RPA4 aa 216-261. The exact sequence is proprietary.
Sequence:EGKSI HELRAQLCDL SVKAIKEAID YLTVEGHIYP TVDREHFKSA D
Database link: Q13156 -
Positive control
- HEK293T, Raw264.7 and PC12 whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.097% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab200742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
Target
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Function
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
Tissue specificity
Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels. -
Cellular localization
Nucleus. Localizes to DNA repair foci after DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 29935 Human
- Omim: 300767 Human
- SwissProt: Q13156 Human
- Unigene: 659349 Human
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Alternative names
- HSU24186 antibody
- MGC120333 antibody
- MGC120334 antibody
see all
Images
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Western blot - Anti-RPA4 antibody - C-terminal (ab200742)All lanes : Anti-RPA4 antibody - C-terminal (ab200742)
Lane 1 : HEK293T whole cell lysate
Lane 2 : Raw264.7 whole cell lysate
Lane 3 : PC12 whole cell lysate
Predicted band size: 29 kDa
Protocols
Datasheets and documents
References
ab200742 has not yet been referenced specifically in any publications.
