Overview

  • Product name

    Anti-RPA4 antibody - C-terminal
  • Description

    Rabbit polyclonal to RPA4 - C-terminal
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of RPA4 protein.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RPA4 aa 216-261. The exact sequence is proprietary.
    Sequence:

    EGKSI HELRAQLCDL SVKAIKEAID YLTVEGHIYP TVDREHFKSA D


    Database link: Q13156

  • Positive control

    • HEK293T, Raw264.7 and PC12 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab200742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.

Target

  • Function

    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Tissue specificity

    Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels.
  • Cellular localization

    Nucleus. Localizes to DNA repair foci after DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSU24186 antibody
    • MGC120333 antibody
    • MGC120334 antibody
    • p30 antibody
    • Replication factor A protein 4 antibody
    • Replication protein A 30 kDa subunit antibody
    • RF A antibody
    • RF-A protein 4 antibody
    • RFA antibody
    • RFA4_HUMAN antibody
    • RP A antibody
    • RP-A p30 antibody
    • RPA antibody
    • RPA4 antibody
    see all

Images

  • All lanes : Anti-RPA4 antibody - C-terminal (ab200742)

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : Raw264.7 whole cell lysate
    Lane 3 : PC12 whole cell lysate

    Predicted band size: 29 kDa

References

ab200742 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 10 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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