Anti-RPA4 antibody - C-terminal (ab200742)
Key features and details
- Rabbit polyclonal to RPA4 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RPA4 antibody - C-terminal -
Description
Rabbit polyclonal to RPA4 - C-terminal -
Host species
Rabbit -
Specificity
Detects endogenous levels of RPA4 protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RPA4 aa 216-261. The exact sequence is proprietary.
Sequence:EGKSI HELRAQLCDL SVKAIKEAID YLTVEGHIYP TVDREHFKSA D
Database link: Q13156 -
Positive control
- HEK293T, Raw264.7 and PC12 whole cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.097% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab200742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
Target
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Function
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
Tissue specificity
Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels. -
Cellular localization
Nucleus. Localizes to DNA repair foci after DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 29935 Human
- Omim: 300767 Human
- SwissProt: Q13156 Human
- Unigene: 659349 Human
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Alternative names
- HSU24186 antibody
- MGC120333 antibody
- MGC120334 antibody
see all
Images
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Western blot - Anti-RPA4 antibody - C-terminal (ab200742)All lanes : Anti-RPA4 antibody - C-terminal (ab200742)
Lane 1 : HEK293T whole cell lysate
Lane 2 : Raw264.7 whole cell lysate
Lane 3 : PC12 whole cell lysate
Predicted band size: 29 kDa
Datasheets and documents
References (0)
ab200742 has not yet been referenced specifically in any publications.
