Key features and details
- Rabbit polyclonal to RPA70
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPA70 antibody
See all RPA70 primary antibodies
DescriptionRabbit polyclonal to RPA70
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
Immunogen was a synthetic peptide, which represented a portion of human replication protein A1, 70kDa encoded within exon 12 (LocusLink ID 6117).
- Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified using the peptide immobilized on solid support.
Our Abpromise guarantee covers the use of ab12320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
FunctionPlays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Sequence similaritiesBelongs to the replication factor A protein 1 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
- Information by UniProt
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RPA70 with ab12320 at 1/1000 (1 µg/ml). Detection: DAB.
Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells. Antibody: ab12320 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells.
Antibody: ab12320 used at the indicated concentrations.
Detection: Chemiluminescence with a 10 second exposure.
ab12320 has been referenced in 3 publications.
- Scarzello AJ et al. A Mutation in the SH2 domain of STAT2 prolongs tyrosine phosphorylation of STAT1 and promotes type I IFN-induced apoptosis. Mol Biol Cell 18:2455-62 (2007). WB ; Human . PubMed: 17442890
- McCabe N et al. Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res 66:8109-15 (2006). PubMed: 16912188
- Saeki H et al. Suppression of the DNA repair defects of BRCA2-deficient cells with heterologous protein fusions. Proc Natl Acad Sci U S A 103:8768-73 (2006). WB ; Human . PubMed: 16731627