Key features and details
- Rabbit polyclonal to RPA70
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPA70 antibody
See all RPA70 primary antibodies
DescriptionRabbit polyclonal to RPA70
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 369 and 616 of Human RPA70 (NP_002936).
- 293T, A431, HepG2, Molt-4, and Raji cell lysates. H1299 and HeLaS3 whole cell lysate. HeLa cells for IF. Cal27 xenograft for IHC.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 68 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/200.|
FunctionPlays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Sequence similaritiesBelongs to the replication factor A protein 1 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
- Information by UniProt
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All lanes : Anti-RPA70 antibody (ab97338) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLaS3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA70 antibody (ab97338) at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA70 antibody (ab97338) at 1/100 dilution.
ab97338 has been referenced in 2 publications.
- Hustedt N et al. Control of homologous recombination by the HROB-MCM8-MCM9 pathway. Genes Dev 33:1397-1415 (2019). PubMed: 31467087
- Marin-Vicente C et al. RRP6/EXOSC10 is required for the repair of DNA double-strand breaks by homologous recombination. J Cell Sci 128:1097-107 (2015). PubMed: 25632158