Product nameAnti-RPA70 antibody [EPR3473]
See all RPA70 primary antibodies
DescriptionRabbit monoclonal [EPR3473] to RPA70
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human RPA70 (UniProt P27694).
- A431 and HeLa cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Sequence similaritiesBelongs to the replication factor A protein 1 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
- Information by UniProt
- Dmrpa1 antibody
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All lanes : Anti-RPA70 antibody [EPR3473] (ab133364) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
ab133364 has not yet been referenced specifically in any publications.