Anti-RPB3 antibody [1Y26] (ab202893)
Key features and details
- Mouse monoclonal [1Y26] to RPB3
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-RPB3 antibody [1Y26]
See all RPB3 primary antibodies -
Description
Mouse monoclonal [1Y26] to RPB3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment corresponding to Saccharomyces cerevisiae RPB3.
Database link: P16370 -
Positive control
- Saccharomyces cerevisiae lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: 100% PBS
0.22 µm filtered -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1Y26 -
Isotype
IgG1 -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. -
Sequence similarities
Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- DNA directed RNA polymerase II 33 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit C antibody
- DNA directed RNA polymerase II subunit RPB3 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (4)
ab202893 has been referenced in 4 publications.
- Baig MS et al. Dynamic sumoylation of promoter-bound general transcription factors facilitates transcription by RNA polymerase II. PLoS Genet 17:e1009828 (2021). PubMed: 34587155
- García-Martínez J et al. Recruitment of Xrn1 to stress-induced genes allows efficient transcription by controlling RNA polymerase II backtracking. RNA Biol N/A:1-17 (2020). PubMed: 33258404
- Tudek A et al. A Nuclear Export Block Triggers the Decay of Newly Synthesized Polyadenylated RNA. Cell Rep 24:2457-2467.e7 (2018). PubMed: 30157437
- Mirón-García MC et al. The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner. PLoS Genet 9:e1003297 (2013). PubMed: 23459708