Overview

  • Product name

    Anti-RPB3 antibody [1Y26]
    See all RPB3 primary antibodies
  • Description

    Mouse monoclonal [1Y26] to RPB3
  • Host species

    Mouse
  • Tested applications

    Suitable for: ChIP, WB, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment corresponding to Saccharomyces cerevisiae RPB3.
    Database link: P16370

  • Positive control

    • Saccharomyces cerevisiae lysate.
  • General notes

    Previously labelled as POLR2C.

Properties

Applications

Our Abpromise guarantee covers the use of ab202893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.
  • Sequence similarities

    Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Alternative names

    • DNA directed RNA polymerase II 33 kDa polypeptide antibody
    • DNA directed RNA polymerase II subunit C antibody
    • DNA directed RNA polymerase II subunit RPB3 antibody
    • DNA-directed RNA polymerase II 33 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit C antibody
    • DNA-directed RNA polymerase II subunit RPB3 antibody
    • hRPB33 antibody
    • hsRPB3 antibody
    • POLR 2C antibody
    • POLR2 C antibody
    • Polr2c antibody
    • Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa antibody
    • polymerase RNA II DNA directed polypeptide C 33kDa antibody
    • RNA polymerase II polypeptide C antibody
    • RNA polymerase II subunit 3 antibody
    • RNA polymerase II subunit B3 antibody
    • RPB 3 antibody
    • RPB3 antibody
    • RPB3_HUMAN antibody
    • RPB31 antibody
    • RPB33 antibody
    see all

Images

  • All lanes : Anti-RPB3 antibody [1Y26] (ab202893) at 1 µg/ml

    Lane 1 : Saccharomyces cerevisiae lysate
    Lane 2 : Jurkat cell lysate

    Secondary
    All lanes : HRP anti-mouse IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa

References

This product has been referenced in:

  • Tudek A  et al. A Nuclear Export Block Triggers the Decay of Newly Synthesized Polyadenylated RNA. Cell Rep 24:2457-2467.e7 (2018). Read more (PubMed: 30157437) »
See 1 Publication for this product

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