Key features and details
- Rabbit polyclonal to RPB3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPB3 antibody
See all RPB3 primary antibodies
DescriptionRabbit polyclonal to RPB3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
- HeLa, Jurkat and 293T cell lysates.
Previously labelled as POLR2C.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99.01% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab138436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 31 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.
Sequence similaritiesBelongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
- Information by UniProt
- DNA directed RNA polymerase II 33 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit C antibody
- DNA directed RNA polymerase II subunit RPB3 antibody
All lanes : Anti-RPB3 antibody (ab138436) at 1/2000 dilution
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 3 minutes
Immunoprecipitation detection of RPB3 in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138436 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138436 at 1µg/ml. Chemiluminescence with an exposure time of 10 seconds.
ab138436 has been referenced in 2 publications.