Overview

  • Product name

  • Description

    Rabbit polyclonal to RPB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human RPB3 aa 225-275.
    Sequence:

    KPERFY YNVESCGSLR PETIVLSALS GLKKKLSDLQ TQLSHEIQSD VLTIN


    Database link: NP_116558.1

  • Positive control

    • HeLa, Jurkat and 293T cell lysates.
  • General notes

    Previously labelled as POLR2C.

Properties

Applications

Our Abpromise guarantee covers the use of ab138436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 31 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.
  • Sequence similarities

    Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA directed RNA polymerase II 33 kDa polypeptide antibody
    • DNA directed RNA polymerase II subunit C antibody
    • DNA directed RNA polymerase II subunit RPB3 antibody
    • DNA-directed RNA polymerase II 33 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit C antibody
    • DNA-directed RNA polymerase II subunit RPB3 antibody
    • hRPB33 antibody
    • hsRPB3 antibody
    • POLR 2C antibody
    • POLR2 C antibody
    • Polr2c antibody
    • Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa antibody
    • polymerase RNA II DNA directed polypeptide C 33kDa antibody
    • RNA polymerase II polypeptide C antibody
    • RNA polymerase II subunit 3 antibody
    • RNA polymerase II subunit B3 antibody
    • RPB 3 antibody
    • RPB3 antibody
    • RPB3_HUMAN antibody
    • RPB31 antibody
    • RPB33 antibody
    see all

Images

  • All lanes : Anti-RPB3 antibody (ab138436) at 1/2000 dilution

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation detection of RPB3 in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138436 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138436 at 1µg/ml. Chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:

See all 2 Publications for this product

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