Recombinant
RabMAb

Recombinant Anti-RPB3 antibody [EPR13300(B)] - C-terminal (ab181193)

Overview

  • Product name

    Anti-RPB3 antibody [EPR13300(B)] - C-terminal
    See all RPB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13300(B)] to RPB3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human RPB3 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P19387

  • Positive control

    • Jurkat, HeLa and 293T cell lysates; Human fetal kidney tissue lysate.
  • General notes

     

     

    Previously labelled as POLR2C.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.
  • Sequence similarities

    Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA directed RNA polymerase II 33 kDa polypeptide antibody
    • DNA directed RNA polymerase II subunit C antibody
    • DNA directed RNA polymerase II subunit RPB3 antibody
    • DNA-directed RNA polymerase II 33 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit C antibody
    • DNA-directed RNA polymerase II subunit RPB3 antibody
    • hRPB33 antibody
    • hsRPB3 antibody
    • POLR 2C antibody
    • POLR2 C antibody
    • Polr2c antibody
    • Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa antibody
    • polymerase RNA II DNA directed polypeptide C 33kDa antibody
    • RNA polymerase II polypeptide C antibody
    • RNA polymerase II subunit 3 antibody
    • RNA polymerase II subunit B3 antibody
    • RPB 3 antibody
    • RPB3 antibody
    • RPB3_HUMAN antibody
    • RPB31 antibody
    • RPB33 antibody
    see all

Images

  • All lanes : Anti-RPB3 antibody [EPR13300(B)] - C-terminal (ab181193) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Human fetal kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 31 kDa

References

ab181193 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181193.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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