Key features and details
- Goat polyclonal to RPB7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPB7 antibody
DescriptionGoat polyclonal to RPB7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Zebrafish
- Human brain cerebellum lysate
Previously labelled as POLR2G.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab93352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.
Sequence similaritiesBelongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
- Information by UniProt
- DNA directed RNA polymerase II 19 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit G antibody
- DNA directed RNA polymerase II subunit RPB7 antibody
Anti-RPB7 antibody (ab93352) at 3 µg/ml + Human brain cerebellum lysate
Predicted band size: 19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
ab93352 has not yet been referenced specifically in any publications.