Overview

  • Product name

  • Description

    Rabbit polyclonal to RPC39
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human RPC39 aa 1-214.
    Database link: Q9H1D9

  • Positive control

    • HeLa whole cell lysate
  • General notes

    Previously labelled as POLR3F.

Properties

Applications

Our Abpromise guarantee covers the use of ab151495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similarities

    Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA directed RNA polymerase III 39 kDa polypeptide antibody
    • DNA directed RNA polymerase III 39 kDa polypeptide F antibody
    • DNA directed RNA polymerase III subunit F antibody
    • DNA directed RNA polymerase III subunit RPC6 antibody
    • DNA directed RNA polymerases III 39 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit F antibody
    • DNA-directed RNA polymerase III subunit RPC6 antibody
    • MGC13517 antibody
    • Polr3f antibody
    • Polymerase (RNA) III (DNA directed) polypeptide F antibody
    • RNA polymerase III 39 kDa subunit antibody
    • RNA polymerase III C39 subunit antibody
    • RNA polymerase III subunit C6 antibody
    • RPC 39 antibody
    • RPC 6 antibody
    • RPC39 antibody
    • RPC6 antibody
    • RPC6_HUMAN antibody
    see all

Images

  • Anti-RPC39 antibody (ab151495) at 1/1000 dilution + HeLa nucleus lysate at 30 µg

    Predicted band size: 36 kDa



    10% SDS PAGE

References

ab151495 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
ChIP
Sample
Human Cell lysate - whole cell (Monocytic cell line)
Negative control
IgG - RNAPII intragenic region
Specification
Monocytic cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1%
Positive control
5S rDNA - tRNA Asp - U6

Abcam user community

Verified customer

Submitted Apr 29 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up