DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
DNA-directed RNA polymerase III subunit C antibody
DNA-directed RNA polymerase III subunit rpc3 antibody
Polymerase (RNA) III (DNA directed) polypeptide C (62kD) antibody
RNA polymerase C subunit 3 antibody
RNA polymerase III 62 kDa subunit antibody
RNA polymerase III C62 subunit antibody
RNA polymerase III subunit C3 antibody
Western blot - Anti-RPC62 antibody (ab112057)
All lanes : Anti-RPC62 antibody (ab112057) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Immunoprecipitation of RPC62. 1 mg of HeLa Whole cell
lysate IP in which 20% of IP was loaded. Blotted with rabbit anti-RPC62 antibody (ab112057) at 0.4 µg/ml. Lane 1- RPC62 was immunoprecipitated by rabbit anti- RPC62 antibody (ab112057) at 6 mcg/mg lysate; Lane 2- RPC62 was immunoprecipitated by an rabbit anti- RPC62 antibody,
that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure times of 3 minutes.