Overview

  • Product name
    Anti-RPC62 antibody
    See all RPC62 primary antibodies
  • Description
    Chicken polyclonal to RPC62
  • Host species
    Chicken
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: HRCY KSIAN LIERR QFET KENKRL LEK SQRVEAI IA SMQATGAE E AQLQEIEEM ITAPERQQLE TLKRNVNKL D ASEIQVDE TI FLLESYI ECT MKRQ, corresponding to amino acids 437-534 of Human RPC62

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab26185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 61 kDa.
ICC 1/200.

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similarities
    Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DNA directed III 62 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit C antibody
    • DNA-directed RNA polymerase III subunit rpc3 antibody
    • POLR3C antibody
    • Polymerase (RNA) III (DNA directed) polypeptide C (62kD) antibody
    • RNA polymerase C subunit 3 antibody
    • RNA polymerase III 62 kDa subunit antibody
    • RNA polymerase III C62 subunit antibody
    • RNA polymerase III subunit C3 antibody
    • RPC3 antibody
    • RPC3_HUMAN antibody
    • RPC62 antibody
    see all

References

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab26185? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-RPC62 antibody

 Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa)
Loading amount
2 µg
Specification
HeLa
Treatment
formaldehyde 1%, 5 min
Blocking step
Tryed: 2% BSA, 5% milk, Roche blocking buffer, Pierce blocking buffer, Millipore special blocking buffer for chicken IgY as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
Username

Dr. Svitlana Melnik

Verified customer

Submitted Mar 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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