Anti-RPC62 antibody (ab88243)
Key features and details
- Mouse polyclonal to RPC62
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPC62 antibody -
Description
Mouse polyclonal to RPC62 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RPC62. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: HeLa nuclear extract, RPC62 transfected 293T cell lysate ICC/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa).
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa). |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. -
Sequence similarities
Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10623 Human
- SwissProt: Q53F76 Human
- SwissProt: Q9BT08 Human
- SwissProt: Q9BUI4 Human
- Unigene: 591457 Human
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Alternative names
- DNA directed III 62 kDa polypeptide antibody
- DNA-directed RNA polymerase III subunit C antibody
- DNA-directed RNA polymerase III subunit rpc3 antibody
see all
Images
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Anti-RPC62 antibody (ab88243) at 1 µg/ml + HeLa nuclear extract at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 61 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RPC62 antibody (ab88243) at 1 µg/ml
Lane 1 : RPC62 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 61 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted? -
ab88243, at 10 µg/ml, staining human RPC62 in HeLa cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab88243 has not yet been referenced specifically in any publications.