• Product name

    Anti-RPE65 antibody [EPR7024(N)] - C-terminal
    See all RPE65 primary antibodies
  • Description

    Rabbit monoclonal [EPR7024(N)] to RPE65 - C-terminal
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPE65 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16518

  • Positive control

    • Mouse eyeball, rat eyeball and Y79 lysates
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab175936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 61 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
    • Tissue specificity

      Retinal pigment epithelium specific.
    • Involvement in disease

      Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
      Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
    • Sequence similarities

      Belongs to the carotenoid oxygenase family.
    • Post-translational

      Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A).
    • Cellular localization

      Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • All-trans-retinyl-palmitate hydrolase antibody
      • LCA 2 antibody
      • LCA2 antibody
      • Leber congenital amaurosis antibody
      • mRPE 65 antibody
      • mRPE65 antibody
      • p63 antibody
      • rd 12 antibody
      • rd12 antibody
      • Retinal pigment epithelium specific 61 kDa protein antibody
      • Retinal pigment epithelium specific 65 kDa protein antibody
      • Retinal pigment epithelium specific protein antibody
      • Retinal pigment epithelium specific protein 65kDa antibody
      • Retinal pigment epithelium-specific 65 kDa protein antibody
      • Retinitis pigmentosa 20 antibody
      • Retinoid isomerohydrolase antibody
      • Retinol isomerase antibody
      • RP 20 antibody
      • RP20 antibody
      • RPE 65 antibody
      • RPE65 antibody
      • RPE65_HUMAN antibody
      • sRPE 65 antibody
      • sRPE65 antibody
      see all


    • Western blot analysis on immunoprecipitation pellet from mouse eyeball lysate labeling RPE65 with ab175936 at 1/10 dilution

    • All lanes : Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936) at 1/1000 dilution

      Lane 1 : Mouse eyeball lysate
      Lane 2 : Rat eyeball lysate
      Lane 3 : Y79 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 61 kDa


    This product has been referenced in:

    • Zhou J  et al. Correlations between Photodegradation of Bisretinoid Constituents of Retina and Dicarbonyl Adduct Deposition. J Biol Chem 290:27215-27 (2015). WB . Read more (PubMed: 26400086) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Western blot
    Mouse Tissue lysate - whole (RPE, retina)
    Gel Running Conditions
    Reduced Denaturing (12% BisTris Gel)
    Loading amount
    10 µg
    RPE, retina
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jul 17 2019

    Western blot
    Human Tissue lysate - whole (RPE lysates)
    Gel Running Conditions
    Reduced Denaturing (12% BisTris)
    Loading amount
    10 µg
    RPE lysates
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jul 17 2019

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