Recombinant Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)
![Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)](https://www.abcam.com/ps/products/175/ab175936/Images/ab175936-203043-anti-rpe65-antibody-epr7024n-c-terminal-immunoprecipitation.jpg "Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7024(N)] to RPE65 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-RPE65 antibody [EPR7024(N)] - C-terminal
See all RPE65 primary antibodies -
Description
Rabbit monoclonal [EPR7024(N)] to RPE65 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPE65 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q16518 -
Positive control
- Mouse eyeball, rat eyeball and Y79 lysates
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7024(N) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab175936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 61 kDa. | |
| IP | 1/10 - 1/100. |
Target
-
Function
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. -
Tissue specificity
Retinal pigment epithelium specific. -
Involvement in disease
Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the carotenoid oxygenase family. -
Post-translational
modificationsPalmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). -
Cellular localization
Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. - Information by UniProt
-
Database links
- Entrez Gene: 6121 Human
- Entrez Gene: 19892 Mouse
- Entrez Gene: 89826 Rat
- Omim: 180069 Human
- SwissProt: Q16518 Human
- SwissProt: Q91ZQ5 Mouse
- SwissProt: O70276 Rat
- Unigene: 2133 Human
see all -
Alternative names
- All-trans-retinyl-palmitate hydrolase antibody
- LCA 2 antibody
- LCA2 antibody
see all
Images
-
Immunoprecipitation - Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)
Western blot analysis on immunoprecipitation pellet from mouse eyeball lysate labeling RPE65 with ab175936 at 1/10 dilution
-
![Western blot - Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)](https://www.abcam.com/ps/products/175/ab175936/Images/ab175936-203042-anti-rpe65-antibody-epr7024n-c-terminal-western-blot.jpg)
Western blot - Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)All lanes : Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936) at 1/1000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lane 3 : Y79 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa -
![Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)](https://www.abcam.com/ps/products/175/ab175936/Images/ab175936-3-benefits-of-recombinant-antibodies.png)
Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)
Protocols
Datasheets and documents
References (1)
ab175936 has been referenced in 1 publication.
- Zhou J et al. Correlations between Photodegradation of Bisretinoid Constituents of Retina and Dicarbonyl Adduct Deposition. J Biol Chem 290:27215-27 (2015). WB . PubMed: 26400086
