Application
Western blot
Sample
Human Tissue lysate - whole (RPE lysates)
Gel Running Conditions
Reduced Denaturing (12% BisTris)
Loading amount
10 µg
Specification
RPE lysates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: ECL Rabbit IgG, HRP-linked whole Ab from donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 26 kDa
Abcam user community
Verified customer
Submitted Jul 17 2019