Overview

  • Product name
    Anti-RPF2 antibody [EP10252]
    See all RPF2 primary antibodies
  • Description
    Rabbit monoclonal [EP10252] to RPF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RPF2 aa 1-100. The exact sequence is proprietary.
    Database link: Q9H7B2

  • Positive control
    • HeLa, 293T, Jurkat and A549 lysates; Human kidney cell carcinoma tissue; 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP10252
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50.
ICC/IF 1/100.

Target

  • Sequence similarities
    Belongs to the RPF2 family.
    Contains 1 Brix domain.
  • Cellular localization
    Nucleus > nucleolus. Associated with the nucleolus in an RNA-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA397G5.4 antibody
    • Brix domain containing 1 antibody
    • Brix domain containing protein 1 antibody
    • Brix domain-containing protein 1 antibody
    • BXDC1 antibody
    • Homolog of Rpf2 antibody
    • Ribosomal processing factor 2 homolog antibody
    • Ribosome biogenesis protein RPF2 homolog antibody
    • Ribosome production factor 2 homolog (S. cerevisiae) antibody
    • Ribosome production factor 2 homolog antibody
    • RPF2 antibody
    • RPF2_HUMAN antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling RPF2 with ab180604 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution (left panel). Dapi stain (right panel).

  • Immunohistochemical analysis of paraffin-embedded Human kidney cell carcinoma labeling RPF2 with ab180604 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • All lanes : Anti-RPF2 antibody [EP10252] (ab180604) at 1/20000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

References

ab180604 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180604.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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