Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling RPF2 with ab180604 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution (left panel). Dapi stain (right panel).
Immunohistochemical analysis of paraffin-embedded Human kidney cell carcinoma labeling RPF2 with ab180604 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Western blot - Anti-RPF2 antibody [EP10252] (ab180604)
All lanes : Anti-RPF2 antibody [EP10252] (ab180604) at 1/20000 dilution
Lane 1 : 293T cell lysate Lane 2 : HeLa cell lysate Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 36 kDa Observed band size: 36 kDa