Overview

  • Product name

    Anti-RPGRIP1 antibody
  • Description

    Rabbit polyclonal to RPGRIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 536-566 of Human RPGRIP1.

  • Positive control

    • Human breast carcinoma tissue; MDA-MB231 cells; HL60, K562, MDA-MB435 and Jurkat cell line lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135832 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 147, 103 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization

    Cell projection > cilium. Situated between the axonemal microtubules and the plasma membrane.
  • Database links

  • Alternative names

    • CORD13 antibody
    • LCA6 antibody
    • Retinitis pigmentosa GTPase regulator interacting protein 1 antibody
    • RGI1 antibody
    • RGRIP antibody
    • RPGR interacting protein antibody
    • RPGR interacting protein 1 antibody
    • RPGR1_HUMAN antibody
    • RPGRIP antibody
    • RPGRIP1d antibody
    • X linked retinitis pigmentosa GTPase regulator interacting protein 1 antibody
    see all

Images

  • All lanes : Anti-RPGRIP1 antibody (ab135832) at 1/100 dilution

    Lane 1 : HL60 cell line lysate
    Lane 2 : K562 cell line lysate
    Lane 3 : MDA-MB435 cell line lysate
    Lane 4 : Jurkat cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 147, 103 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelling RPGRIP1 with ab135832 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow cytometry analysis of MDA-MB231 cells labelling RPGRIP1 with ab135832 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab135832 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab135832.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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