Key features and details
- Rabbit polyclonal to RPIA/PRI
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPIA/PRI antibody
See all RPIA/PRI primary antibodies
DescriptionRabbit polyclonal to RPIA/PRI
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Pig
Recombinant fragment corresponding to Human RPIA/PRI aa 82-311.
KKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIPT SFQARQLILQYGLTLSDLDRPEIDLAIDGADEVDADLNLIKGGGGCLTQE KIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRAVSQK FGGVVELRMAVNKAGPVVTDNGNFILDWKFDRVHKWSEVNTAIKMIPGVV DTGLFINMAERVYFGMQDGSVNMREKPFC
Database link: P49247
- WB: Mouse kidney lysate. IHC-P: Human placenta and gastric cancer tissues.
This product was previously labelled as RPIA
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/20 - 1/200.|
PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.
Involvement in diseaseDefects in RPIA are the cause of ribose 5-phosphate isomerase deficiency (RPID) [MIM:608611]. A patient has been described with a deficiency of ribose 5-phosphate isomerase who presented with leukoencephalopathy and peripheral neuropathy. Proton magnetic resonance spectroscopy of the brain revealed a highly elevated level of the polyols ribitol and D-arabitol, which were subsequently also found in high concentrations in body fluids. Deficient activity of RPIA, one of the pentose phosphate pathway enzymes, has been demonstrated in fibroblasts.
Sequence similaritiesBelongs to the ribose 5-phosphate isomerase family.
- Information by UniProt
- EC 220.127.116.11 antibody
- MGC103524 antibody
- Phosphoriboisomerase A antibody
Paraffin-embedded human gastric cancer tissue stained for RPIA/PRI using ab229967 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for RPIA/PRI using ab229967 at 1/100 dilution in immunohistochemical analysis.
Anti-RPIA/PRI antibody (ab229967) at 1/1000 dilution + Mouse kidney tissue
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229967 has not yet been referenced specifically in any publications.