Recombinant Anti-RPIA/PRI antibody [EPR12961] (ab181235)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12961] to RPIA/PRI
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RPIA/PRI antibody [EPR12961]
See all RPIA/PRI primary antibodies -
Description
Rabbit monoclonal [EPR12961] to RPIA/PRI -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, 293 and NIH:OVCAR-3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12961 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1. -
Involvement in disease
Defects in RPIA are the cause of ribose 5-phosphate isomerase deficiency (RPID) [MIM:608611]. A patient has been described with a deficiency of ribose 5-phosphate isomerase who presented with leukoencephalopathy and peripheral neuropathy. Proton magnetic resonance spectroscopy of the brain revealed a highly elevated level of the polyols ribitol and D-arabitol, which were subsequently also found in high concentrations in body fluids. Deficient activity of RPIA, one of the pentose phosphate pathway enzymes, has been demonstrated in fibroblasts. -
Sequence similarities
Belongs to the ribose 5-phosphate isomerase family. - Information by UniProt
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Database links
- Entrez Gene: 22934 Human
- Omim: 180430 Human
- SwissProt: P49247 Human
- Unigene: 469264 Human
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Alternative names
- EC 5.3.1.6 antibody
- MGC103524 antibody
- Phosphoriboisomerase A antibody
see all
Images
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All lanes : Anti-RPIA/PRI antibody [EPR12961] (ab181235) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : NIH:OVCAR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa -
Anti-RPIA/PRI antibody [EPR12961] (ab181235) at 1/5000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab181235 has been referenced in 4 publications.
- Zhu L et al. Myostatin Deficiency Enhances Antioxidant Capacity of Bovine Muscle via the SMAD-AMPK-G6PD Pathway. Oxid Med Cell Longev 2022:3497644 (2022). PubMed: 35663205
- Li Y et al. APC/CCDH1 synchronizes ribose-5-phosphate levels and DNA synthesis to cell cycle progression. Nat Commun 10:2502 (2019). PubMed: 31175280
- Buj R et al. Suppression of p16 Induces mTORC1-Mediated Nucleotide Metabolic Reprogramming. Cell Rep 28:1971-1980.e8 (2019). PubMed: 31433975
- Persi E et al. Systems analysis of intracellular pH vulnerabilities for cancer therapy. Nat Commun 9:2997 (2018). PubMed: 30065243