Overview

  • Product name

    Anti-RPIA/PRI antibody [EPR12961]
    See all RPIA/PRI primary antibodies
  • Description

    Rabbit monoclonal [EPR12961] to RPIA/PRI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RPIA/PRI aa 1-150. The exact sequence is proprietary.
    Database link: P49247

  • Positive control

    • HepG2, 293 and NIH:OVCAR-3 cell lysates.
  • General notes

     

     

     This product was previously labelled as RPIA

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • Pathway

    Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.
  • Involvement in disease

    Defects in RPIA are the cause of ribose 5-phosphate isomerase deficiency (RPID) [MIM:608611]. A patient has been described with a deficiency of ribose 5-phosphate isomerase who presented with leukoencephalopathy and peripheral neuropathy. Proton magnetic resonance spectroscopy of the brain revealed a highly elevated level of the polyols ribitol and D-arabitol, which were subsequently also found in high concentrations in body fluids. Deficient activity of RPIA, one of the pentose phosphate pathway enzymes, has been demonstrated in fibroblasts.
  • Sequence similarities

    Belongs to the ribose 5-phosphate isomerase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 5.3.1.6 antibody
    • MGC103524 antibody
    • Phosphoriboisomerase A antibody
    • Phosphoriboisomerase antibody
    • Ribose 5 phosphate isomerase antibody
    • ribose 5-phosphate epimerase antibody
    • ribose 5-phosphate isomerase A antibody
    • Ribose-5-phosphate isomerase antibody
    • RPI antibody
    • rpiA antibody
    • RPIA_HUMAN antibody
    • zgc:103524 antibody
    see all

Images

  • All lanes : Anti-RPIA/PRI antibody [EPR12961] (ab181235) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : NIH:OVCAR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa

  • Anti-RPIA/PRI antibody [EPR12961] (ab181235) at 1/5000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa

References

This product has been referenced in:

See 1 Publication for this product

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