Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12961] to RPIA/PRI
- Suitable for: WB
- Reacts with: Human
Product nameAnti-RPIA/PRI antibody [EPR12961]
See all RPIA/PRI primary antibodies
DescriptionRabbit monoclonal [EPR12961] to RPIA/PRI
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human RPIA/PRI aa 1-150. The exact sequence is proprietary.
Database link: P49247
- HepG2, 293 and NIH:OVCAR-3 cell lysates.
This product was previously labelled as RPIA
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.
Involvement in diseaseDefects in RPIA are the cause of ribose 5-phosphate isomerase deficiency (RPID) [MIM:608611]. A patient has been described with a deficiency of ribose 5-phosphate isomerase who presented with leukoencephalopathy and peripheral neuropathy. Proton magnetic resonance spectroscopy of the brain revealed a highly elevated level of the polyols ribitol and D-arabitol, which were subsequently also found in high concentrations in body fluids. Deficient activity of RPIA, one of the pentose phosphate pathway enzymes, has been demonstrated in fibroblasts.
Sequence similaritiesBelongs to the ribose 5-phosphate isomerase family.
- Information by UniProt
- EC 126.96.36.199 antibody
- MGC103524 antibody
- Phosphoriboisomerase A antibody
All lanes : Anti-RPIA/PRI antibody [EPR12961] (ab181235) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : NIH:OVCAR-3 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Anti-RPIA/PRI antibody [EPR12961] (ab181235) at 1/5000 dilution + 293 cell lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
ab181235 has been referenced in 3 publications.
- Li Y et al. APC/CCDH1 synchronizes ribose-5-phosphate levels and DNA synthesis to cell cycle progression. Nat Commun 10:2502 (2019). PubMed: 31175280
- Buj R et al. Suppression of p16 Induces mTORC1-Mediated Nucleotide Metabolic Reprogramming. Cell Rep 28:1971-1980.e8 (2019). PubMed: 31433975
- Persi E et al. Systems analysis of intracellular pH vulnerabilities for cancer therapy. Nat Commun 9:2997 (2018). PubMed: 30065243