Anti-RPL10 antibody (ab110371)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RPL10 antibody
See all RPL10 primary antibodies -
Description
Rabbit polyclonal to RPL10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide derived from internal sequence of Human RPL10.
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Positive control
- Extracts from K562 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab110371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 25 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Down-regulated during adipocyte, kidney, and heart differentiation. Note=Two hypomorphic variants with respect to the regulation of the translation process have been found in families affected by autism spectrum disorders, suggesting that aberrant processes of ribosome biosynthesis and translation may play a role in disease mechanisms. -
Cellular localization
Endoplasmic reticulum Cytosolic large ribosomal subunit -
Database links
- Entrez Gene: 6134 Human
- Entrez Gene: 110954 Mouse
- Entrez Gene: 81764 Rat
- Omim: 312173 Human
- SwissProt: P27635 Human
- SwissProt: Q6ZWV3 Mouse
- SwissProt: Q6PDV7 Rat
- Unigene: 534404 Human
see all -
Alternative names
- 60S ribosomal protein L10 antibody
- DKFZp686J1851 antibody
- DXS648 antibody
see all
Images
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Western blot - Anti-RPL10 antibody (ab110371)All lanes : Anti-RPL10 antibody (ab110371) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa
Datasheets and documents
References
ab110371 has not yet been referenced specifically in any publications.
