Product nameAnti-RPL10A antibody
See all RPL10A primary antibodies
DescriptionRabbit polyclonal to RPL10A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Cynomolgus monkey, Orangutan
Synthetic peptide within Human RPL10A aa 50-100. The exact sequence is proprietary.
Database link: P62906
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226381 was affinity purified using an epitope specific to RPL10A immobilized on solid support.
Our Abpromise guarantee covers the use of ab226381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 25 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the ribosomal protein L1P family.
- Information by UniProt
- 60S ribosomal protein L10a antibody
- CSA 19 antibody
- CSA-19 antibody
All lanes : Anti-RPL10A antibody (ab226381) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
RPL10A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226381 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226381 at 0.4 µg/ml.
Lane 1: ab226381 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 75 seconds.
ab226381 has not yet been referenced specifically in any publications.