Product nameAnti-RPL10A antibody [EPR12344]
See all RPL10A primary antibodies
DescriptionRabbit monoclonal [EPR12344] to RPL10A
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human RPL10A. The exact sequence is proprietary.
Database link: P62906
- HepG2 and PC-12 lysates. Human endometrial adenocarcinoma tissue. Jurkat and HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab174318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the ribosomal protein L1P family.
- Information by UniProt
- 60S ribosomal protein L10a antibody
- CSA 19 antibody
- CSA-19 antibody
All lanes : Anti-RPL10A antibody [EPR12344] (ab174318) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-Rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelling RPL10A using ab174318 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells labelling RPL10A with ab174318 at 1/100 dilution (red) and DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized Jurkat cells labeling RPL10A with ab174318 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
This product has been referenced in:
- Megat S et al. Nociceptor Translational Profiling Reveals the Ragulator-Rag GTPase Complex as a Critical Generator of Neuropathic Pain. J Neurosci 39:393-411 (2019). Read more (PubMed: 30459229) »
- Liang XH et al. Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs. Nucleic Acids Res 46:293-313 (2018). Read more (PubMed: 29165591) »