RecombinantRabMAb

Recombinant Anti-RPL10A antibody [EPR12344] (ab174318)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12344] to RPL10A
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPL10A antibody [EPR12344]
    See all RPL10A primary antibodies

  • Description

    Rabbit monoclonal [EPR12344] to RPL10A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human RPL10A. The exact sequence is proprietary.
    Database link: P62906

  • Positive control

    • HepG2 and PC-12 lysates. Human endometrial adenocarcinoma tissue. Jurkat and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12344

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
      Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
      All lanes : Anti-RPL10A antibody [EPR12344] (ab174318) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)

      Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelling RPL10A using ab174318 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (ab174318)
      Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (ab174318)

      Immunofluorescence analysis of HeLa cells labelling RPL10A with ab174318 at 1/100 dilution (red) and DAPI nuclear staining (blue).

    • Flow Cytometry - Anti-RPL10A antibody [EPR12344] (ab174318)
      Flow Cytometry - Anti-RPL10A antibody [EPR12344] (ab174318)

      Flow cytometric analysis of permeabilized Jurkat cells labeling  RPL10A with ab174318 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Anti-RPL10A antibody [EPR12344] (ab174318)
      Anti-RPL10A antibody [EPR12344] (ab174318)

    References (3)

    Publishing research using ab174318? Please let us know so that we can cite the reference in this datasheet.

    ab174318 has been referenced in 3 publications.

    • Megat S  et al. Nociceptor Translational Profiling Reveals the Ragulator-Rag GTPase Complex as a Critical Generator of Neuropathic Pain. J Neurosci 39:393-411 (2019). PubMed: 30459229
    • Fujii K  et al. Decoding the Function of Expansion Segments in Ribosomes. Mol Cell 72:1013-1020.e6 (2018). PubMed: 30576652
    • Liang XH  et al. Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs. Nucleic Acids Res 46:293-313 (2018). PubMed: 29165591

    Customer reviews and Q&As

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    Western blot abreview for Anti-RPL10A antibody [EPR12344]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae)
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient gel)
    Loading amount
    10 µg
    Specification
    S. cerevisiae
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 14 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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