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RecombinantRabMAb

Recombinant Anti-RPL10A antibody [EPR12344] (ab174318)

Reviews (1) Submit a question References (6)
Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Flow Cytometry - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunocytochemistry - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
  • Anti-RPL10A antibody [EPR12344] (ab174318)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12344] to RPL10A
  • Suitable for: Flow Cyt (Intra), ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-RPL10A antibody [EPR12344]
    See all RPL10A primary antibodies

  • Description

    Rabbit monoclonal [EPR12344] to RPL10A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human RPL10A. The exact sequence is proprietary.
    Database link: P62906

  • Positive control

    • WB: Jurkat, HeLa, HepG2 whole cell lysates and Mouse brain, Rat brain, Mouse spleen and Rat spleen lysates Flow cyt: Jurkat cells, ICC: HeLa cells, IHC: rat liver tissue, mouse liver tissue, human colon tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12344

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab174318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10 - 1/100 dilution.
ICC
1/500.

For unpurified use at 1/100 dilution.
WB (1)
1/10000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/10000 - 1/10000 dilution.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250 dilution.
Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10 - 1/100 dilution.
ICC
1/500.

For unpurified use at 1/100 dilution.
WB
1/10000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/10000 - 1/10000 dilution.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250 dilution.
Application notes
Is unsuitable for IP.

Target

Images

  • Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
    Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
    All lanes : Anti-RPL10A antibody [EPR12344] (ab174318) at 1/10000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Mouse spleen lysate
    Lane 7 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

  • Flow Cytometry - Anti-RPL10A antibody [EPR12344] (ab174318)
    Flow Cytometry - Anti-RPL10A antibody [EPR12344] (ab174318)
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling RPL10A with Purified ab174318 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunocytochemistry - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPL10A with Purified ab174318 at 1:500 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling RPL10A with Purified ab174318 at 1:1000 dilution (0.207 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling RPL10A with Purified ab174318 at 1:1000 dilution (0.207 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling RPL10A with Purified ab174318 at 1:1000 dilution (0.207 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Anti-RPL10A antibody [EPR12344] (ab174318)
    Anti-RPL10A antibody [EPR12344] (ab174318)

References (6)

Publishing research using ab174318? Please let us know so that we can cite the reference in this datasheet.

ab174318 has been referenced in 6 publications.

  • Simbriger K  et al. Uncovering memory-related gene expression in contextual fear conditioning using ribosome profiling. Prog Neurobiol 197:101903 (2021). PubMed: 32860876
  • Yang KD  et al. Multi-domain translation between single-cell imaging and sequencing data using autoencoders. Nat Commun 12:31 (2021). PubMed: 33397893
  • Echavarría-Consuegra L  et al. Manipulation of the unfolded protein response: A pharmacological strategy against coronavirus infection. PLoS Pathog 17:e1009644 (2021). PubMed: 34138976
  • Megat S  et al. Nociceptor Translational Profiling Reveals the Ragulator-Rag GTPase Complex as a Critical Generator of Neuropathic Pain. J Neurosci 39:393-411 (2019). PubMed: 30459229
  • Fujii K  et al. Decoding the Function of Expansion Segments in Ribosomes. Mol Cell 72:1013-1020.e6 (2018). PubMed: 30576652
  • Liang XH  et al. Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs. Nucleic Acids Res 46:293-313 (2018). PubMed: 29165591

Customer reviews and Q&As

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Western blot abreview for Anti-RPL10A antibody [EPR12344]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
10 µg
Specification
S. cerevisiae
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Aug 14 2019

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