Anti-RPL11 antibody (ab79352)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameAnti-RPL11 antibody
See all RPL11 primary antibodies -
DescriptionRabbit polyclonal to RPL11
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
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Species reactivityReacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Cow, Cat, Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RPL11.
Read Abcam's proprietary immunogen policy (Peptide available as ab88221.) -
Positive control
- This antibody gave a positive signal in human liver and human brain tissue lysates and in the following whole cell lysates: HepG2; HeLa; Jurkat; Caco 2; HEK293. It also gave a positive control in human pancreas tissue sections.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab79352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa). | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21046615 | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionBinds to 5S ribosomal RNA (By similarity). Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML.
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Involvement in diseaseDefects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7) [MIM:612562]. DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
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Sequence similaritiesBelongs to the ribosomal protein L5P family.
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Cellular localizationNucleus > nucleolus.
- Information by UniProt
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Database links
- Entrez Gene: 6135 Human
- Entrez Gene: 67025 Mouse
- Entrez Gene: 362631 Rat
- Entrez Gene: 415229 Zebrafish
- Omim: 604175 Human
- SwissProt: P62913 Human
- SwissProt: Q9CXW4 Mouse
- SwissProt: P62914 Rat
see all -
Alternative names
- 60S ribosomal protein L11 antibody
- Cell growth inhibiting protein 34 antibody
- Cell growth inhibiting protein34 antibody
see all
Images
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Western blot - Anti-RPL11 antibody (ab79352)All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 30 kDa, 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
RPL11 contains a number of potential phosphorylation and acetylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunoprecipitation - Anti-RPL11 antibody (ab79352)RPL11 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RPL11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79352.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 22kDa: RPL11 -
- Anti-RPL11 antibody (ab79352)IHC image of ab79352 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79352, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-RPL11 antibody (ab79352)All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 6 : Human hepatocellular carcinoma cell line at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
References
This product has been referenced in:
- Martins T et al. TGFß/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands. Open Biol 7:N/A (2017). Read more (PubMed: 28123053) »
- Paolini NA et al. A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism. Am J Hum Genet 100:506-522 (2017). Read more (PubMed: 28257692) »
