Anti-RPL11 antibody (ab79352)

Reviews (3)Q&A (1)References (9)

Overview

  • Product name

    Anti-RPL11 antibody
    See all RPL11 primary antibodies

  • Description

    Rabbit polyclonal to RPL11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Cow, Cat, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human RPL11 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88221)

  • Positive control

    • This antibody gave a positive signal in human liver and human brain tissue lysates and in the following whole cell lysates: HepG2; HeLa; Jurkat; Caco 2; HEK293. It also gave a positive control in human pancreas tissue sections.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 21046615
IP Use at an assay dependent concentration.

Target

  • Function

    Binds to 5S ribosomal RNA (By similarity). Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML.

  • Involvement in disease

    Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7) [MIM:612562]. DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.

  • Sequence similarities

    Belongs to the ribosomal protein L5P family.

  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 60S ribosomal protein L11 antibody
    • Cell growth inhibiting protein 34 antibody
    • Cell growth inhibiting protein34 antibody
    • CLL associated antigen KW 12 antibody
    • CLL associated antigen KW12 antibody
    • CLL-associated antigen KW-12 antibody
    • DBA 7 antibody
    • DBA7 antibody
    • GIG 34 antibody
    • GIG34 antibody
    • L11 antibody
    • Ribosomal protein L11 antibody
    • RL11_HUMAN antibody
    • RPL 11 antibody
    • Rpl11 antibody
    see all

Images

  • Western blot - Anti-RPL11 antibody (ab79352)
    Western blot - Anti-RPL11 antibody (ab79352)
    All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 30 kDa, 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.



    RPL11 contains a number of potential phosphorylation and acetylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunoprecipitation - Anti-RPL11 antibody (ab79352)
    Immunoprecipitation - Anti-RPL11 antibody (ab79352)
    RPL11 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RPL11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79352.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 22kDa: RPL11
  • - Anti-RPL11 antibody (ab79352)
    - Anti-RPL11 antibody (ab79352)
    IHC image of ab79352 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79352, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-RPL11 antibody (ab79352)
    Western blot - Anti-RPL11 antibody (ab79352)
    All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
    Lane 6 : Human hepatocellular carcinoma cell line at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes

References

This product has been referenced in:

  • Martins T  et al. TGFß/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands. Open Biol 7:N/A (2017). Read more (PubMed: 28123053) »
  • Paolini NA  et al. A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism. Am J Hum Genet 100:506-522 (2017). Read more (PubMed: 28257692) »
See all 9 Publications for this product

Publishing research using ab79352? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-RPL11 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - other (yeast cells)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
yeast cells
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 27 2017

Western blot abreview for Anti-RPL11 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human ES cell ribosome gradient)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
human ES cell ribosome gradient
Blocking step
Li-COR blocking solution as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 13 2017

Western blot abreview for Anti-RPL11 antibody

 Good
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 23 2013

Question

Our customer is willing to test ab79352 in IP with rabbit samples (the customer verified 100% homology of the desired protein to human). Are you interested in this type of the information for the AbTrial program? (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)? They will also add the positive control.


Read More
Answer


Expiration date: 27-Mar-13


I am very pleased to hear you would like to accept our offer and test ab79352 in rabbit samples.
To redeem this offer, please submit an Abreview for rabbit samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com