Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-143 of Human RPL12 (UniProt P30050).

  • Positive control

    • Raji whole cell lysate; Human Gastric carcinoma and Mouse muscle tissues
  • General notes

    Store undiluted.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Images

  • Anti-RPL12 antibody (ab127533) at 1/1000 dilution + Raji whole cell
    lysate at 30 µg

    Predicted band size: 18 kDa



    12% SDS-PAGE
  • ab127533, at 1/100 dilution, staining RPL12 in paraffin-embedded Human gastric carcinoma tissue by Immunohistochemistry.
  • ab127533, at 1/100 dilution, staining RPL12 in paraffin-embedded Mouse muscle tissue by Immunohistochemistry.

References

ab127533 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer



I am very pleased to hear you would like to accept our offer and test Anti-RPL12 antibody (ab127533) in pig tissue. This code will give you: 1 freeprimary before the expiration date. To redeem this offer, please submit an Abreview forpig tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in our products.


I have BLASTed the sequence against the human immunogen, andI am delighted to inform you that the two proteins from both species have a 100% similarity, and therefore, all of our three antibodies against this target should cross-react with pig.

https://www.abcam.com/index.html?datasheet=74714 (or use the following: https://www.abcam.com/index.html?datasheet=74714).

https://www.abcam.com/index.html?datasheet=123954 (or use the following: https://www.abcam.com/index.html?datasheet=123954).

https://www.abcam.com/index.html?datasheet=127533 (or use the following: https://www.abcam.com/index.html?datasheet=127533).


Therefore, I can offer a discount off a future purchase if you buyeither ab74714, ab123954and/or ab127533now, test it inpig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody for every tested antibody.


If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab74714, ab123954 and/or ab127533 in pig. I will then send a discount code. This code must be issued before purchasing ab74714, ab123954 and/or ab127533 so please wait for my reply before ordering.

2. Purchase ab74714, ab123954 and/or ab127533 either by phone, fax, or online (www.abcam.com).

3. Test it in pig.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab74714, ab123954 and/or ab127533 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.

We have 3 antibodies for the protein RPL12 all of which were raised against a portion of the human protein sequence.

I have aligned the human and pig sequences and unfortunately, no homology was found (0%). Thus, antibodies raised against a human RPL12 sequence will not be able to bind to the pig protein.

You would need an antibody that was raised against the pig protein for the antibody to be specific for pig.

I am sorry to not have better news for you.
But please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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