Anti-RPL12 antibody (ab157130)

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Overview

  • Product name

    Anti-RPL12 antibody
    See all RPL12 primary antibodies

  • Description

    Rabbit polyclonal to RPL12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis

  • Immunogen

    Recombinant fragment corresponding to a region between residues 115-165 of Human RPL12 (NP_000967.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Jurkat, HeLa, 293T and mouse NIH3T3 whole cell lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-RPL12 antibody (ab157130)
      Western blot - Anti-RPL12 antibody (ab157130)
      All lanes : Anti-RPL12 antibody (ab157130) at 0.4 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 18 kDa


      Exposure time: 3 minutes

    References

    Publishing research using ab157130? Please let us know so that we can cite the reference in this datasheet.

    ab157130 has been referenced in 2 publications.

    • Oliver KE  et al. Slowing ribosome velocity restores folding and function of mutant CFTR. J Clin Invest 129:5236-5253 (2019). PubMed: 31657788
    • Veit G  et al. Ribosomal Stalk Protein Silencing Partially Corrects the ?F508-CFTR Functional Expression Defect. PLoS Biol 14:e1002462 (2016). WB ; Human . PubMed: 27168400

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