Overview

  • Product name
    Anti-RPL12 antibody
    See all RPL12 primary antibodies
  • Description
    Rabbit polyclonal to RPL12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region between residues 115-165 of Human RPL12 (NP_000967.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat, HeLa, 293T and mouse NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-RPL12 antibody (ab157130)
      Western blot - Anti-RPL12 antibody (ab157130)
      All lanes : Anti-RPL12 antibody (ab157130) at 0.4 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 18 kDa


      Exposure time: 3 minutes

    References

    This product has been referenced in:
    • Veit G  et al. Ribosomal Stalk Protein Silencing Partially Corrects the ?F508-CFTR Functional Expression Defect. PLoS Biol 14:e1002462 (2016). WB ; Human . Read more (PubMed: 27168400) »

    See 1 Publication for this product

    Publishing research using ab157130? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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