Anti-RPL12 antibody (ab157130)

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Western blot - Anti-RPL12 antibody (ab157130)

    Key features and details

    • Rabbit polyclonal to RPL12
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-RPL12 antibody
      See all RPL12 primary antibodies

    • Description

      Rabbit polyclonal to RPL12

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis

    • Immunogen

      Recombinant fragment corresponding to a region between residues 115-165 of Human RPL12 (NP_000967.1).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Jurkat, HeLa, 293T and mouse NIH3T3 whole cell lysates.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: 99% Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab157130 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
    Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 18 kDa.

    Target

    Images

    • Western blot - Anti-RPL12 antibody (ab157130)
      Western blot - Anti-RPL12 antibody (ab157130)
      All lanes : Anti-RPL12 antibody (ab157130) at 0.4 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 18 kDa


      Exposure time: 3 minutes

    References (2)

    Publishing research using ab157130? Please let us know so that we can cite the reference in this datasheet.

    ab157130 has been referenced in 2 publications.

    • Oliver KE  et al. Slowing ribosome velocity restores folding and function of mutant CFTR. J Clin Invest 129:5236-5253 (2019). PubMed: 31657788
    • Veit G  et al. Ribosomal Stalk Protein Silencing Partially Corrects the ?F508-CFTR Functional Expression Defect. PLoS Biol 14:e1002462 (2016). WB ; Human . PubMed: 27168400

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