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    rpl12-antibody-ab157130.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-RPL12 antibody (ab157130)

  • Datasheet
  • SDS
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Western blot - Anti-RPL12 antibody (ab157130)

    Key features and details

    • Rabbit polyclonal to RPL12
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-RPL12 antibody
      See all RPL12 primary antibodies
    • Description

      Rabbit polyclonal to RPL12
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis
    • Immunogen

      Recombinant fragment corresponding to a region between residues 115-165 of Human RPL12 (NP_000967.1).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Jurkat, HeLa, 293T and mouse NIH3T3 whole cell lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: 99% Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • Translation
      • Ribosome

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • Jurkat whole cell lysate (ab30128)
      • NIH/3T3 whole cell lysate (ab7179)
      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant Human RPL12 protein (ab140558)
    • Related Products

      • Recombinant Human RPL12 protein (ab140558)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab157130 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
    Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 18 kDa.

    Target

    • Function

      Binds directly to 26S ribosomal RNA.
    • Sequence similarities

      Belongs to the ribosomal protein L11P family.
    • Target information above from: UniProt accession P30050 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6136 Human
      • Entrez Gene: 269261 Mouse
      • Entrez Gene: 499782 Rat
      • Entrez Gene: 685320 Rat
      • Omim: 180475 Human
      • SwissProt: P30050 Human
      • SwissProt: P35979 Mouse
      • SwissProt: P23358 Rat
      • Unigene: 408054 Human
      • Unigene: 250030 Mouse
      • Unigene: 381297 Mouse
      • Unigene: 128979 Rat
      • Unigene: 203094 Rat
      see all
    • Alternative names

      • 60S ribosomal protein L12 antibody
      • L12 antibody
      • Ribosomal protein L12 antibody
      • RL12_HUMAN antibody
      • rpl12 antibody
      see all

    Images

    • Western blot - Anti-RPL12 antibody (ab157130)
      Western blot - Anti-RPL12 antibody (ab157130)
      All lanes : Anti-RPL12 antibody (ab157130) at 0.4 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 18 kDa


      Exposure time: 3 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab157130? Please let us know so that we can cite the reference in this datasheet.

    ab157130 has been referenced in 2 publications.

    • Oliver KE  et al. Slowing ribosome velocity restores folding and function of mutant CFTR. J Clin Invest 129:5236-5253 (2019). PubMed: 31657788
    • Veit G  et al. Ribosomal Stalk Protein Silencing Partially Corrects the ?F508-CFTR Functional Expression Defect. PLoS Biol 14:e1002462 (2016). WB ; Human . PubMed: 27168400

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab157130.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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