Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL13A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-197 of Human RPL13A (NP_036555).

  • Positive control

    • 293T and HeLa whole cell lysate (ab150035); HeLa cells; A431, H1299, HepG2, Molt-4 and Raji cell lines This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance

    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative polyA signals have been observed. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in its second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • Database links

  • Alternative names

    • 23 kD highly basic protein antibody
    • 23 kDa highly basic protein antibody
    • 60S ribosomal protein L13a antibody
    • L13A antibody
    • P198 antibody
    • Ribosomal protein L13a antibody
    • RPL 13A antibody
    • Tissue specific transplantation antigen 1 antibody
    • Transplantation antigen P198 antibody
    • TSTA1 antibody
    • Tstap198 7 antibody
    • Tum antigen antibody
    • Tum P198 antigen antibody
    see all

Images

  • IHC image of RPL13A staining in Human normal pancreas (exocrine glandular cells) formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96074, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-RPL13A antibody (ab96074) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 24 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96074 at 1/200 dilution. Lower image is merged with DNA probe.

References

ab96074 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - other (Huh7)
Loading amount
50 µg
Specification
Huh7
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 12 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (ST (Swine Testis))
Specification
ST (Swine Testis)
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-100 05% in PBS 1X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 25 2011

Application
Western blot
Sample
Pig Cell lysate - whole cell (ST (Swine Testis))
Loading amount
68000 cells
Specification
ST (Swine Testis)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 22 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh7)
Specification
Huh7
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-100 05% in PBS 1X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 22 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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