Product nameAnti-RPL13A antibody
See all RPL13A primary antibodies
DescriptionRabbit polyclonal to RPL13A
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Zebrafish
Recombinant fragment, corresponding to a region within amino acids 1-197 of Human RPL13A (NP_036555).
- 293T and HeLa whole cell lysate (ab150035); HeLa cells; A431, H1299, HepG2, Molt-4 and Raji cell lines This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/3000. Predicted molecular weight: 24 kDa.|
|ICC/IF||1/100 - 1/200.|
RelevanceRibosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative polyA signals have been observed. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in its second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
- 23 kD highly basic protein antibody
- 23 kDa highly basic protein antibody
- 60S ribosomal protein L13a antibody
IHC image of RPL13A staining in Human normal pancreas (exocrine glandular cells) formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96074, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-RPL13A antibody (ab96074) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96074 at 1/200 dilution. Lower image is merged with DNA probe.
ab96074 has not yet been referenced specifically in any publications.