Anti-RPL13A antibody (ab96074)
Key features and details
- Rabbit polyclonal to RPL13A
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-RPL13A antibody
See all RPL13A primary antibodies -
Description
Rabbit polyclonal to RPL13A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Zebrafish
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-197 of Human RPL13A (NP_036555).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab96074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 24 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Relevance
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative polyA signals have been observed. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in its second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
Database links
- Entrez Gene: 767925 Cow
- Entrez Gene: 23521 Human
- Entrez Gene: 22121 Mouse
- Entrez Gene: 780432 Pig
- Entrez Gene: 317646 Rat
- SwissProt: Q3SZ90 Cow
- SwissProt: P40429 Human
- SwissProt: P19253 Mouse
see all -
Alternative names
- 23 kD highly basic protein antibody
- 23 kDa highly basic protein antibody
- 60S ribosomal protein L13a antibody
see all
Images
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Western blot - Anti-RPL13A antibody (ab96074)All lanes : Anti-RPL13A antibody (ab96074) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RPL13A antibody (ab96074)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96074 at 1/200 dilution. Lower image is merged with DNA probe.
Protocols
References (0)
ab96074 has not yet been referenced specifically in any publications.
