Application
Western blot
Sample
Pig Cell lysate - whole cell (ST (Swine Testis))
Loading amount
68000 cells
Specification
ST (Swine Testis)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 3% milk in TBS with tween 20 at 0,05%
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit (whole molecule) peroxidase conjugate
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20
Detection
Detection method
Immobilom
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 32 kDa Non-specific: 17, 7 kDa
Positive control
1. ST Cell extract
2. ST Cell extract
2. ST Cell extract
Abcam user community
Verified customer
Submitted Mar 22 2011