Product nameAnti-RPL17 antibody
See all RPL17 primary antibodies
DescriptionRabbit polyclonal to RPL17
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cat, Pig, Monkey, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 1-184 of Human RPL17 (Uniprot ID P18621).
- HeLa and HepG2 whole cell lysate (ab7900); HeLa cells; HBL435 xenograft tissue; 293T, A431, H1299, MOLT4 and Raji cell lines.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityExpressed in pancreas, lung, colon, cystic duct, gall bladder, kidney and liver. Expressed at high levels in the well differentiated pancreatic tumor cell lines HPAF, Colo 357 and Capan-1, the moderately differentiated pancreatic tumor cell lines T3M4, AsPc-1 and BxPc-3, the poorly differentiated pancreatic tumor cell line Mia Paca, and the pancreatic tumor cell lines of undefined differentiation status Panc 89 and SW 979. Expressed at lower levels in the poorly differentiated pancreatic tumor cell lines HGC 25 and Panc 1.
Sequence similaritiesBelongs to the ribosomal protein L22P family.
- Information by UniProt
- 60S ribosomal protein L17 antibody
- 60S ribosomal protein L23 antibody
- FLJ92089 antibody
All lanes : Anti-RPL17 antibody (ab155781) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa
12% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling RPL17 with ab155781 at 1/200 dilution. Bottom image: costaining with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human HBL435 xenograft, labeling RPL17 with ab155781 at 1/500 dilution.
ab155781 has not yet been referenced specifically in any publications.