Anti-RPL18A antibody (ab188131)
Key features and details
- Rabbit polyclonal to RPL18A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL18A antibody
See all RPL18A primary antibodies -
Description
Rabbit polyclonal to RPL18A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human RPL18A aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q02543 -
Positive control
- IHC-P: Human pancreas tissue. WB: Human cell line RT4 and U-251 MG. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Relevance
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL18A belongs to the L18AE family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U68 snoRNA, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
Cellular localization
Cytosolic large ribosomal subunit -
Database links
- Entrez Gene: 6142 Human
- Entrez Gene: 76808 Mouse
- Entrez Gene: 290641 Rat
- Omim: 604178 Human
- SwissProt: Q02543 Human
- SwissProt: P62717 Mouse
- SwissProt: P62718 Rat
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Alternative names
- 2510019J09Rik antibody
- 60S ribosomal protein L18a antibody
- L18A antibody
see all
Images
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All lanes : Anti-RPL18A antibody (ab188131) at 1/250 dilution
Lane 1 : Human cell line RT-4
Lane 2 : Human cell line U-251MG sp
Predicted band size: 21 kDa -
Immunohistochemical analysis of Human pancreas tissue labeling RPL18A using ab188131 at a 1/2500 dilution.
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U-2 OS cells stained for RPL18A (green) using ab188131 at 2 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab188131 has not yet been referenced specifically in any publications.