Anti-RPL22 antibody (ab77720)
Key features and details
- Rabbit polyclonal to RPL22
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL22 antibody
See all RPL22 primary antibodies -
Description
Rabbit polyclonal to RPL22 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Pig, Zebrafish, Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human RPL22 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab87822) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa; HEK293; Jurkat.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
Target
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Sequence similarities
Belongs to the ribosomal protein L22e family. - Information by UniProt
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Database links
- Entrez Gene: 6146 Human
- Entrez Gene: 100504863 Mouse
- Entrez Gene: 19934 Mouse
- Entrez Gene: 81768 Rat
- Omim: 180474 Human
- SwissProt: P35268 Human
- SwissProt: P67984 Mouse
- SwissProt: P47198 Rat
see all -
Alternative names
- 60S ribosomal protein L22 antibody
- EAP antibody
- EBER associated protein antibody
see all
Images
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All lanes : Anti-RPL22 antibody (ab77720) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab77720 has been referenced in 2 publications.
- Riepe C et al. Double stranded DNA breaks and genome editing trigger loss of ribosomal protein RPS27A. FEBS J 289:3101-3114 (2022). PubMed: 34914197
- Hannan KM et al. Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway. Cell Rep 41:111571 (2022). PubMed: 36323262