Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Pig, Zebrafish, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human RPL22 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87822)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa; HEK293; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab77720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

Images

  • All lanes : Anti-RPL22 antibody (ab77720) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa
    Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.

References

ab77720 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Chicken Cell lysate - whole cell (T CELLS)
Gel Running Conditions
Non-reduced Denaturing (12 %)
Loading amount
1e+006 cells
Specification
T CELLS
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ahmed Kheimar

Verified customer

Submitted Jun 28 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Forebrain)
Loading amount
10 µg
Specification
Forebrain
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 14 2013

Answer

I am sorry that you did not recieve my original response to your question. I emailed you a reply on the 24th January 2012. Please find a copy of the email I sent below:
There are no references associated with the antibody, however we do have a western blot image for this antibody on our webpage. Under our Abpromise we do guarantee that this antibody will work in western blot on human samples and we predict that it will work in Mouse, Rat, Horse, Chicken, Pig, Zebrafish and Cynomolgus Monkey. If you would like to use the antibody in one of the predicted only species then it may qualify for one of our testing discounts, you can find out more about this by clicking on the link below:
https://www.abcam.com/index.htmlpageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
Also the immunogen sequence for this antibody is *************.
If there is anything else that I can help you with, please let me know.

Read More

Answer

Thank you for contacting Abcam.
There are no references associated with the antibody, however we do have a western blot image for this antibody on our webpage. Under our Abpromise we do guarantee that this antibody will work in western blot on human samples and we predict that it will work in Mouse, Rat, Horse, Chicken, Pig, Zebrafish and Cynomolgus Monkey. If you would like to use the antibody in one of the predicted only species then it may qualify for one of our testing discounts, you can find out more about this by clicking on the link below:
https://www.abcam.com/index.htmlpageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
Also the immunogen sequence for this antibody is *************************
If there is anything else that I can help you with, please let me know.

Read More

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