Overview

  • Product name
  • Description
    Goat polyclonal to RPL23
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    KECADLWPRIASN

    , corresponding to C terminal amino acids 123-135 of Human RPL23 (NP_000969.1).

  • Positive control
    • Human colorectal cancer lysate, NIH3T3 and HeLa cell line lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156813 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).

Target

Images

  • Immunohistochemical analysis of paraffin embedded Human Liver staining RPL23 with ab156813 (5µg/ml). Steamed antigen retrieval with citrate buffer pH 6.
  • Immunohistochemical analysis of paraffin embedded Human Tonsil staining RPL23 with ab156813 (5µg/ml). Steamed antigen retrieval with citrate buffer pH 6.
  • Anti-RPL23 antibody (ab156813) at 0.1 µg/ml + Human colorectal cancer lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 16 kDa (why is the actual band size different from the predicted?)

References

ab156813 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Drosophila melanogaster Cell lysate - whole cell (S2 Drosophila cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
200000 cells
Specification
S2 Drosophila cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Marianthi Kiparaki

Verified customer

Submitted May 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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