Product nameAnti-RPL23 antibody
See all RPL23 primary antibodies
DescriptionRabbit polyclonal to RPL23
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Zebrafish, Orangutan
Synthetic peptide within Human RPL23 aa 90-140. The exact sequence is proprietary.
Database link: P62829
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264369 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 15 kDa.|
Sequence similaritiesBelongs to the ribosomal protein L14P family.
- Information by UniProt
- 60S ribosomal protein L17 antibody
- 60S ribosomal protein L23 antibody
- L23 antibody
All lanes : Anti-RPL23 antibody (ab264369) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 15 kDa
Exposure time: 1 second
RPL23 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab264369 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264369 at 1 μg/ml.
Lane 1: ab264369 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time: 10 seconds.
ab264369 has not yet been referenced specifically in any publications.